Ductal epithelial cells cultured from human foetal epididymis and vas deferens: relevance to sterility in cystic fibrosis.
نویسندگان
چکیده
A tissue culture system for epithelial cells derived from male human foetal genital ducts has been established. The cells show morphological and biochemical characteristics of ductal epithelial cells, and can be passaged and maintained in culture for considerable periods of time. These cells will provide a suitable system for investigating, by electrophysiological, biochemical and molecular biological methods, the cause of sterility in cystic fibrosis.
منابع مشابه
Ovine male genital duct epithelial cells differentiate in vitro and express functional CFTR and ENaC.
To investigate the biology of the male genital duct epithelium, we have established cell cultures from the ovine vas deferens and epididymis epithelium. These cells develop tight junctions, high transepithelial electrical resistance, and a lumen-negative transepithelial potential difference as a sign of active transepithelial ion transport. In epididymis cultures the equivalent short-circuit cu...
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ورودعنوان ژورنال:
- Journal of cell science
دوره 92 ( Pt 4) شماره
صفحات -
تاریخ انتشار 1989